Additional file 3.

Spontaneous degradation of 4MU-Sialic acid compounds. All three fluorescent reporter compounds, 4-methyl-umbelliferyl (4MU), linked to Neu5Ac, Neu5Gc, or 2-keto-3-deoxynononic acid (Kdn) spontaneously degrade during 1 h incubation at 37°C. Notably 4MU-Neu5Gc is the least stable compound. The fluorescent resulting from spontaneous degradation of the reporter compounds was accounted as background and was either subtracted from the results (Figure 5A) or presented as background (Figure 5B).

Format: JPEG Size: 625KB Download file

Cohen et al. Virology Journal 2013 10:321   doi:10.1186/1743-422X-10-321