Additional file 1.

Characterization of mucus samples. (A) Mucin composition in the HSM preparation is comparable to that of the human airway epithelium submucosal glands. (B) Amino acid composition was analyzed by gas chromatography mass spectrometry, and (C) monosaccharide composition was analyzed by high performance anion exchange chromatography with Pulsed Amperometric Detection (HPAEC-PAD). (D) The Sialic acid (Sia) content of PSM and HSM was analyzed by DMB-HPLC. Sias in HSM are linked Neu5Acα2-6GalNAc or Neu5Acα2-3Galβ1-3GalNAc [14], in PSM Sias are found as Neu5Acα2-6GalNAc or Neu5Gcα2-6GalNAc [17]. (E) Boiling of HSM samples for 10 min inactivates bacterial sialidase activity, which is typically found in saliva preparations.

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Cohen et al. Virology Journal 2013 10:321   doi:10.1186/1743-422X-10-321