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Open Access Short report

Purified hepatitis B virus induces human Mesangial cell proliferation and extracellular matrix expression In Vitro

Zongli Diao1, Jiaxiang Ding1, Chenghong Yin2, Liyan Wang1 and Wenhu Liu1*

Author Affiliations

1 Department of Nephrology, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An Road, Beijing 100050, China

2 Department of Infectious Disease, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An Road, Beijing 100050, China

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Virology Journal 2013, 10:300  doi:10.1186/1743-422X-10-300

Published: 4 October 2013

Abstract

Background

Hepatitis B virus (HBV) induces proliferation of human mesangial cells (HMCs), and extracellular matrix expression through the deposition of immune complexes in renal tissue. However, it is unclear whether HBV can directly affect HMCs. In this study, the effects of purified HBV on HMC proliferation and extracellular matrix expression in vitro was determined.

Findings

HBV was purified using sucrose density gradient centrifugation. HMCs were co-cultured with purified HBV (104–106 copies/ml) for 48 h, and cell proliferation determined using 5-ethynyl-2′-deoxyuridine immunofluorescence assays. After HMCs were co-cultured with 106 copies/ml purified HBV for 0, 12, 24, 36 and 48 h, expression of type IV collagen and fibronectin was measured using enzyme-linked immunosorbent assays. Three titers of purified HBV (104, 105, and 106 copies/ml) induced HMC proliferation, with the proportion of increases in cell numbers at 24.7 ± 4.3, 31.2 ± 9.4, and 36.8 ± 7.5%, respectively. All these increases were significantly higher than those for the control group (13.6 ± 4.2%) (All p < 0.05). Purified HBV (106 copies/ml) significantly increased the levels of type IV collagen and fibronectin in supernatants compared with the control group at 12 and 48 h (All p < 0.05).

Conclusions

Purified HBV can directly promote HMC proliferation and expression of type IV collagen and fibronectin, and could be involved in the pathogenesis of HBV-associated glomerulonephritis.

Keywords:
Hepatitis B virus; Human mesangial cells; Proliferation; Type IV collagen; Fibronectin