Overview of EV71 replication and molecular mechanisms of potential inhibitors. EV71 binds to its receptor on host cell and releases the genomic RNA into cytoplasm. Neutralizing antibodies bind to the receptor-binding capsid protein VP1 and prevent the virus-receptor interaction. VP1-binding small molecules, such as pleconaril, BTA798, and BPR0Z-194, can also interfere in virus entry. In cytoplasm, viral RNA can be targeted by virus-specific siRNAs for suppressing virus replication. A single large polyprotein is first synthesized and then cleaved by viral proteases 2A and 3C to form functional proteins. Rupintrivir and Compound 1 can inhibit 3C protease activity to prevent maturation cleveage.
Kuo and Shih Virology Journal 2013 10:28 doi:10.1186/1743-422X-10-28