Effect on porcine GRP78 expression by PEDV E expression in cultured IEC cells. (A) The level of GRP78 expression was determined by western blot with the mouse monoclonal antibodies against GRP78 in all cell lines. β-actin was used as an internal loading control. (B) Total RNA was extracted from cells expressing either GFP alone, GFP-E or untransfected cells. Real-time PCR analysis of GRP78 mRNA levels were normalized to the corresponding CT value for porcine β-actin mRNA. The results are means ± SD and representative of three independent experiments.
Xu et al. Virology Journal 2013 10:26 doi:10.1186/1743-422X-10-26