Detection and subcellular localization of GFP-E fusion protein in IEC cells. (A) Western blot analyses of expression products of GFP-E fusion protein in IEC cells. The cells were subjected to Western blot using anti-GFP antibody. Proteins were isolated from whole extracts of the stable cell lines expressing GFP-E protein and control cells. (B) The cells were subjected to Western blot using porcine anti-PEDV antibody. (C) Detection of GFP-E fusion protein subcellular localization in IEC cells by confocal microscopy. All the cell lines were stained by Hoechst33342 and ER-Tracker™ Red. Merged images showed co-localization of GFP-E protein primarily in the ER, with small amounts translocating in the nucleus. Bar = 20 μm for all the figures.
Xu et al. Virology Journal 2013 10:26 doi:10.1186/1743-422X-10-26