Table 1

Sequences and references for primers used for RT and PCR reactions
Virus designation Primer names and sequences (5′ to 3′) Gene target Amplicon size References
EMCV P1 = CCC TAC CTC ACG GAA TGG GGC AAA G 3D polymerase gene 286 bp [13]
P2 = GGT GAG AGC AAG CCT CGC AAA GAC AG
EMCV P9 = ATC AAG ACT CCA GCT CTC GGG GTC A VP3/VP1 capsid gene 855 bp [26]
P10 = TGC CTA TCT CAC CTG CCC CCT GGA G
Enterovirus RotbartFor = CCT CCG GCC CCT GAA TGC GGC TAAT 5′ non-coding region 153 bp [25]
RotbartRev = ACC GAC GAA TAC CAC TGT TA
Enterovirus EV2 = TCC GGC CCC TGA ATG CGG CTA ATC C 5′ non-coding region 113 bp [24]
EV1 = ACA CGG ACA CCC AAA GTA GTC GGT CC

Yeo et al.

Yeo et al. Virology Journal 2013 10:248   doi:10.1186/1743-422X-10-248

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