Evaluation of the brain Iba1 levels of different human prion diseases by Western blots. A. Temporal lobes. Same amounts of 10% homogenates from three FFI, one sCJD (Case 1), one G114V gCJD and normal control were separated onto 15% SDS-PAGE and blotted with Iba1 or β-actin specific mAbs. The quantitative analyses of the relative gray values are showed on the right. The results are calculated from three blots and presented as mean ± SD. Statistical differences are illustrated as **P<0.01. B. Different brain regions. Same amounts of 10% homogenates of six regions, including thalamus, cingulate gyrus, frontal lobe, parietal lobe, occipital lobe and temporal lobe, from three FFI and the G114V gCJD cases were comparatively assayed. Same amounts of 10% homogenates of temporal lobe from one sCJD (Case 2) and normal one were loaded as control. Same amounts of 10% homogenates of frontal lobe, parietal lobe and occipital lobe from sCJD (Case 1) were also comparatively assayed with that of normal control (low panel). Molecular markers are indicated on the right.
Shi et al. Virology Journal 2013 10:216 doi:10.1186/1743-422X-10-216