PrPSc deposits in the tissues of temporal lobes of different human prion diseases. A. Western blots. Same amounts (10 μl) of 10% brain homogenates from three FFI, two sCJD and one G114V gCJD were loaded into 15% SDS-PAGE, after treated with 20 μg/ml PK (+) or without PK (−). Molecular markers are indicated on the right. B. Immunohistochemical assays. Tissue sections from three FFI, one sCJD (Case 1), one G114V gCJD and one normal control were strained with PrP mAb 3F4 after treated with 6M GdnHCl. Different prion diseases and normal control are indicated above. Scale bar, 20 μm.
Shi et al. Virology Journal 2013 10:216 doi:10.1186/1743-422X-10-216