The first complete genome sequence of HCV-1a from Pakistan and a phylogenetic analysis with complete genomes from the rest of the world
1 Division of Molecular Virology & Molecular Diagnostics, National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan
2 Department of Biotechnology and Informatics, BUITEMS, Quetta, Pakistan
Virology Journal 2013, 10:211 doi:10.1186/1743-422X-10-211Published: 27 June 2013
Here, we report the first patient derived hepatitis C virus (HCV) complete genome from Pakistan as is not available from this region of the world.
Comprehensive evolutionary and phylogenetic analyses were conducted. The comparison was made in order to identify evolutionary and molecular phylogenetic relationships among HCV strains belonging to genotype 1a. The evolutionary divergence analysis for nucleotide and amino acid sequences, conducted by equal input model, suggested that evolutionary nucleotide and amino acid distances showed that the HCV Pakistani strain was genetically far from Denmark strain (0.29400 nt, 0.819646 aa) and near to German strain (0.06557 nt, 0.139449 aa), respectively.
The current study will help to understand phylogenetic relationship of circulating Pakistani isolates.