Table 3

Comparison of HA assay response from VLPs produced in Mammalian and Insect cells and chicken egg derived influenza virus
Production System Influenza strain Sample preparation HA titer Contamination Normalized Concentration (HA units/ml)
VLPs from Sf9 - baculovirus infection [16] HA:A/California/07/2009 (H1N1) Cell culture media 16 HA units/50 μl baculovirus 108 BV/ml 16 HA units/50 μl
M1:A/Udorn/307/1972 (H3N2)
VLPs from HighFive - baculovirus infection [16] HA:A/California/07/2009 (H1N1) Cell culture media 16 HA units/50 μl baculovirus 106 BV/ml 16 HA units/50 μl
M1:A/Udorn/307/1972 (H3N2)
VLPs from HEK 293 T - plasmid transfection [17] HA/NA/M1: A/Puerto Rico/8/34 (H1N1) Purified VLPs concentrated 200 times 32 HA units/50 μl none 0.16 HA units/50 μl
VLPs from HEK 293 T - baculovirus transduction [17] HA/NA/M1 Purified VLPs concentrated 200 times 128 HA units/50 μl none 0.64 HA units/50 μl
A/Puerto Rico/8/34 (H1N1)
Influenza virus Chicken Eggs [59] H1N1 A/PR/8/1934 Purified virus 256 HA units/50 μl none 256 HA units/50 μl

This is a rough comparison, as each system used a different source of red blood cells (chicken, turkey, human) at different percentages (0.5-1.25%).

Thompson et al.

Thompson et al. Virology Journal 2013 10:141   doi:10.1186/1743-422X-10-141

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