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Open Access Research

Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri

Weicheng Ren12, Haixia Chen3, Tristan Renault4, Yuyong Cai1, Changming Bai1, Chongming Wang1* and Jie Huang1

Author Affiliations

1 Maricultural Organism Disease Control and Pathogenic Molecular Biology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao, 266071, China

2 Department of Rheumatology and Inflammation, University of Gothenburg, Gothenburg, 40530, Sweden

3 Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, 40530, Sweden

4 Ifremer, Unité Santé, Génétique et Microgiologie des Mollusques, Laboratoire de Génétique et Pathologie des Mollusques Marins, La Tremblade, 17390, France

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Virology Journal 2013, 10:110  doi:10.1186/1743-422X-10-110

Published: 8 April 2013

Abstract

Background

Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV.

Results

The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats.

Conclusions

Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.

Keywords:
Acute viral necrosis virus (AVNV); Herpesvirus; OsHV-1; Genome