Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri
1 Maricultural Organism Disease Control and Pathogenic Molecular Biology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao, 266071, China
2 Department of Rheumatology and Inflammation, University of Gothenburg, Gothenburg, 40530, Sweden
3 Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, 40530, Sweden
4 Ifremer, Unité Santé, Génétique et Microgiologie des Mollusques, Laboratoire de Génétique et Pathologie des Mollusques Marins, La Tremblade, 17390, France
Virology Journal 2013, 10:110 doi:10.1186/1743-422X-10-110Published: 8 April 2013
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV.
The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats.
Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.