Open Access Open Badges Research

Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from giant panda and raccoon dogs in China

Ling Guo1, Shao-lin Yang1, Cheng-dong Wang2, Rong Hou3, Shi-jie Chen4, Xiao-nong Yang5, Jie Liu1, Hai-bo Pan1, Zhong-xiang Hao1, Man-li Zhang1, San-jie Cao1 and Qi-gui Yan16*

Author Affiliations

1 College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China

2 China Conservation and Research Center for the Giant Panda, Ya’an, China

3 Chengdu Research Base for Giant Panda Breeding, Chengdu, China

4 Enery-exit Inspection and Quarantine of Sichuan Province, Chengdu, China

5 Southwest University for Nationalities, Chengdu, China

6 Key laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, China

For all author emails, please log on.

Virology Journal 2013, 10:109  doi:10.1186/1743-422X-10-109

Published: 8 April 2013



Canine distemper virus (CDV) infects a variety of carnivores, including wild and domestic Canidae. In this study, we sequenced and phylogenetic analyses of the hemagglutinin (H) genes from eight canine distemper virus (CDV) isolates obtained from seven raccoon dogs (Nyctereutes procyonoides) and a giant panda (Ailuropoda melanoleuca) in China.


Phylogenetic analysis of the partial hemagglutinin gene sequences showed close clustering for geographic lineages, clearly distinct from vaccine strains and other wild-type foreign CDV strains, all the CDV strains were characterized as Asia-1 genotype and were highly similar to each other (91.5-99.8% nt and 94.4-99.8% aa). The giant panda and raccoon dogs all were 549Y on the HA protein in this study, irrespective of the host species.


These findings enhance our knowledge of the genetic characteristics of Chinese CDV isolates, and may facilitate the development of effective strategies for monitoring and controlling CDV for wild canids and non-cainds in China.

Canine distemper virus; Haemagglutinin (H) gene; Genotype; Phylogenetic analysis