Additional file 1: Figure S1.
Representative sensograms of IgG b12 SPR data. SPR binding isotherms detailing the interaction of each recombinant trimeric Envgp140 with the monoclonal antibody b12 are displayed. Indicated concentrations of each Envgp140 were flowed over captured b12 at 37°C on CM5 chips as detailed in Materials and Methods. Association rates, dissociation rates, and affinity constants were calculated with BIA evaluation 4.1.1 software (GE/Biacore AB, Uppsala, Sweden). A, ConA; B, ConB; C, ConC; D, Con M; E, ConE; F, ADA; G, PV0.04; H, R2; I, SC42; and J, YU2. Black lines are determined 1:1 Langmuir kinetic fits. RU, resonance units.
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Eugene et al. Virology Journal 2013 10:102 doi:10.1186/1743-422X-10-102