Resolution:
standard / ## Figure 2.
Binding breadth of vaccinated NHPs sera. NHP were vaccinated at weeks 0, 4 and 8 with blood collected 14 days after each vaccination.
Vaccines were formulated with 300 μg of purified protein and Imject® alum and delivered
intramuscularly. A) Sera collected on day 35 was pooled and used to determine total IgG via ELISA for
each vaccine group. Bar values represent the geometric mean titer (+95% confidence
interval) of log2 transformed titers. The endpoint titer is described on the y–axis
and the identified Env_{gp140} trimer antigen is described on the x-axis. B) The unrooted phylogenetic tree was produced using Phylogeny.fr web service and the
14 HIV-1 Env_{gp160} sequences based upon the list of Env_{gp160s} in Table 2 showing. The Env_{gp160s} were from clades A, B, C, E from 1993-2005. The clades are indicated on the tree
and the consensus Env_{gp160s} are circled. C) At day 35 post-vaccination, anti-Env_{gp160} IgG was detected in the serum samples (1:100 dilution) against a panel of primary
Env_{gp120s} from clades A, B, C and E via ELISA. Bar values represent the geometric mean titer
(95% confidence interval) at an OD450. The OD450 values are displayed on the y-axis
and the Env_{gp120s} used are listed on the x-axis. A two-way ANOVA with Bonferroni’s post-test was used
to evaluate Statistical significance between the vaccines for each test antigen. A
p-value of less than 0.05 was considered significant.
Eugene |