Binding breadth of vaccinated NHPs sera. NHP were vaccinated at weeks 0, 4 and 8 with blood collected 14 days after each vaccination. Vaccines were formulated with 300 μg of purified protein and Imject® alum and delivered intramuscularly. A) Sera collected on day 35 was pooled and used to determine total IgG via ELISA for each vaccine group. Bar values represent the geometric mean titer (+95% confidence interval) of log2 transformed titers. The endpoint titer is described on the y–axis and the identified Envgp140 trimer antigen is described on the x-axis. B) The unrooted phylogenetic tree was produced using Phylogeny.fr web service and the 14 HIV-1 Envgp160 sequences based upon the list of Envgp160s in Table 2 showing. The Envgp160s were from clades A, B, C, E from 1993-2005. The clades are indicated on the tree and the consensus Envgp160s are circled. C) At day 35 post-vaccination, anti-Envgp160 IgG was detected in the serum samples (1:100 dilution) against a panel of primary Envgp120s from clades A, B, C and E via ELISA. Bar values represent the geometric mean titer (95% confidence interval) at an OD450. The OD450 values are displayed on the y-axis and the Envgp120s used are listed on the x-axis. A two-way ANOVA with Bonferroni’s post-test was used to evaluate Statistical significance between the vaccines for each test antigen. A p-value of less than 0.05 was considered significant.
Eugene et al. Virology Journal 2013 10:102 doi:10.1186/1743-422X-10-102