Genomic analysis of a newly isolated of Japanese encephalitis virus strain, CQ11-66, from a pediatric patient in China
1 Department of Infectious diseases and Gastroenterology, Children’s Hospital of Chongqing Medical University, No. 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, PR China
2 Ministry of Education Key Laboratory of Child Development and Disorders, No. 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, China
3 Key Laboratory of Pediatrics in Chongqing,CSTC2009CA5002), No. 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, China
4 Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, No 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, China
5 Chongqing Center for Disease Control and Prevention, Chongqing, China
Virology Journal 2013, 10:101 doi:10.1186/1743-422X-10-101Published: 1 April 2013
Japanese encephalitis virus (JEV) is one of the major causative agents of viral encephalitis in East Asia, Southeast Asia and Australia. However, no clinical JEV strain has yet been isolated from JE patients in Chongqing, China. In this study, we report the genomic analysis of a new JEV strain, CQ11-66, isolated from a pediatric patient in Chongqing, China.
Virus isolation was carried out in BHK-21 cells. Nested PCR was used to detect and isolate the JEV strain, and computer analysis of phylogenetic relationships, nucleic acid homology studies and deduction of the amino acid sequence were conducted using ClustalX (1.8) and Mega5 software. The JEV strain CQ11-66 was isolated from patient cerebrospinal fluid. The sequenced genome of CQ11-66 was 10,863 nucleotides in length, whereas other strains, such as SX09S-01, contain 10,965 nucleotides. Sequence comparison of the CQ11-66 polyprotein open reading frame (ORF) with those of 21 other JEV strains revealed that the nucleotide sequence divergence ranged from 1.68% to 18.46%. Sequence analysis of the full-length CQ11-66 E gene sequence with those of 30 other JEV isolates also identified nucleotide divergence, ranging from 1.69% to 18.74%. Phylogenetic analyses indicated that the CQ11-66 strain belonged to genotype III.
JEV genotype III still circulates in Chongqing and it is therefore important for active surveillance of JEV genotype III to be conducted in the pediatric population.